bio rad gene pulser system Search Results


electroporation cuvette  (Bio-Rad)
96
Bio-Rad electroporation cuvette
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Electroporation Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio rad gene pulser xcell  (Bio-Rad)
97
Bio-Rad bio rad gene pulser xcell
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Bio Rad Gene Pulser Xcell, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene pulser mxcell plate electroporation system  (Bio-Rad)
96
Bio-Rad gene pulser mxcell plate electroporation system
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Gene Pulser Mxcell Plate Electroporation System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene pulser electroporation cuvette  (Bio-Rad)
96
Bio-Rad gene pulser electroporation cuvette
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Gene Pulser Electroporation Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genepulser xcelltm  (Bio-Rad)
94
Bio-Rad genepulser xcelltm
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Genepulser Xcelltm, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio rad gene pulser electroporation system  (Bio-Rad)
96
Bio-Rad bio rad gene pulser electroporation system
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Bio Rad Gene Pulser Electroporation System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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genepulser xcell microbial system  (Bio-Rad)
94
Bio-Rad genepulser xcell microbial system
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Genepulser Xcell Microbial System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene pulser cuvettes  (Bio-Rad)
95
Bio-Rad gene pulser cuvettes
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Gene Pulser Cuvettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene pulser xcelltm electroporation system  (Bio-Rad)
94
Bio-Rad gene pulser xcelltm electroporation system
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Gene Pulser Xcelltm Electroporation System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solid state laserstacktm 3i n a tirf laser microscope cube 3i n a gene pulser xcell bio rad  (Bio-Rad)
96
Bio-Rad solid state laserstacktm 3i n a tirf laser microscope cube 3i n a gene pulser xcell bio rad
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Solid State Laserstacktm 3i N A Tirf Laser Microscope Cube 3i N A Gene Pulser Xcell Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pc module  (Bio-Rad)
93
Bio-Rad pc module
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Pc Module, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ice cold electroporation cuvette  (Bio-Rad)
96
Bio-Rad ice cold electroporation cuvette
(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe <t>electroporation</t> (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .
Ice Cold Electroporation Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe electroporation (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .

Journal: bioRxiv

Article Title: Synaptopodin enables directional mechanoadaptation of integrin-based adhesions

doi: 10.64898/2026.01.25.701551

Figure Lengend Snippet: (a, b) Low-magnification immunofluorescence images of Synpo ⁻/⁻ primary podocytes transfected with the synaptopodin long isoform (Synpo-L) using nanofountain probe electroporation (NFP-E) and stained for synaptopodin using an N-terminal-specific antibody (green) (a) and myosin IIA (MyoIIA; purple). (b) Mosaic expression allows direct comparison of transfected and non-transfected cells within the same field. (c, d) Higher magnification of a non-transfected Synpo ⁻/⁻ podocyte showing MyoIIA alone (c) and merged with synaptopodin staining (d) . In the absence of synaptopodin, MyoIIA displays diffuse, disorganized distribution with no discernible periodic structure. (e, f) Higher magnification of a neighboring transfected podocyte showing MyoIIA alone (e) and merged with synaptopodin staining (f) . Following synaptopodin expression, MyoIIA adopts a sarcomeric pattern, forming alternating bands with synaptopodin along stress fibers. This cell-autonomous reorganization demonstrates that synaptopodin is sufficient to organize the actomyosin cytoskeleton into the sarcomere-like structures (SLSs) observed in WT cells and suggests that synaptopodin acts as a structural template for contractile machinery assembly. The single-cell transfection approach provides an internal control, as transfected and non-transfected cells experience identical culture conditions and imaging parameters. Scale bars: 20 μm (a, b) ; 10 μm (c–f) .

Article Snippet: Cells were transferred to a 4 mm electroporation cuvette (Bio-Rad, 1652088) and electroporated using a Gene Pulser Xcell system (Bio-Rad) with the following parameters: 140 V, square wave pulse, 25 ms duration.

Techniques: Immunofluorescence, Transfection, Electroporation, Staining, Expressing, Comparison, Control, Imaging